- Route E trains run clockwise
- Route B trains run counter-clockwise
- At Frankfort High, Route E trains become Route K for the Algonquin Inner Line
- At Frankfort Low, Route B trains become Route C for the Algonquin Inner line
On its way through Bohan, the trains will cross the Humboldt River twice, in connection to Algonquin. Between San Quentin Avenue Station and Frankfort High Station the trains run over a railway bridge south of the Northwood Heights Bridge . Between Frankfort Low Station and Windmill Street Station the trains run underneath the river through a tunnel north of the Northwood Heights Bridge. Although the line runs through the north and eastern sides of Bohan, there are no stations on that part of the island.
The line can be used prior to Algonquin being unlocked in Blow Your Cover, but only between the Windmill and San Quentin stations, which is a very short hop as they are only a few blocks apart.